Hawkeye (Clint Barton) (First and only known appearance).

Ghost Rider (Johnny Blaze) (First and only known appearance).

Captain America (Steve Rogers) (Last appearance).

Some other heroes and anti-heroes aid Venom and Spidey throughout the game, making brief cameos to attack the player's foes.

Carnage (Cletus Kasady) (Final surprise boss) (Last appearance).

Scream (Donna Diego) (First and only known appearance).

Agony (Leslie Gesneria) (First and only known appearance).

Lasher (Ramon Hernandez) (First and only known appearance).

Phage (Carl Mach) (First and only known appearance).

Riot (Trevor Cole) (First and only known appearance).

Life Foundation Symbiotes (First and only known appearance).

Wysper (Jennifer Stewart} (First and only known appearance).

Firearm (First and only known appearance).

Bomblast (First and only known appearance).

Screech (Maxwell Taylor) (First and only known appearance).Ramshot (Samuel Caulkin) (First and only known appearance).Sentry (Curtis Elkins) (First and only known appearance).The Jury (First and only known appearance).

Spider-Man (Peter Parker) (Last appearance).

Along the way, the player(s) can also find and pick up some helpful superhero ally icons that allow you to summon a few of Spidey's greatest allies to your aid including Captain America, Hawk-Eye, Ghost Rider and Dare Devil. The game allows you to punch, kick and swing your way as well as execute combos throughout the streets, the woods and even through the underground complexes towards accomplishing your main goal. As Venom recovers from his wounds, he thus realizes and decides that he must set out and fight them off either alone or with the aid of his old arch-nemesis Spider-Man. As Spider-Man and/or Venom, you must set off and stop the life foundation that has spawned out 5 symbiote offsprings from Venom and plans to bond them together with its soldiers in order to assemble and form a vast deadly army. In this game, 1-2 players can select and take control of Spider-Man and/or his on-and-off again arch-nemesis Venom. The game is based loosely on a mini-series of comics about the Venom saga. Separation Anxiety is a 2D side-scrolling platform-action beat 'em up game featuring several prominent characters from the Marvel Universe and gripping storylines taken from the popular Spider-Man comics.

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